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C22orf33 Polyclonal antibody

C22orf33 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26577-1-AP

Print datasheet

Synonyms

Testis-expressed protein 33, Protein EAN57, EAN57, CIMIP4, Ciliary microtubule inner protein 4

验证数据展示

  • IF Staining of U2OS using 26577-1-AP

    IF Staining of U2OS using 26577-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 26577-1-AP (C22orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human testis using 26577-1-AP

    WB analysis of human testis using 26577-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 26577-1-AP (C22orf33 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 26577-1-AP

    IF Staining of mouse testis using 26577-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C22orf33 antibody (26577-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26577-1-AP targets C22orf33 in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen C22orf33 fusion protein Ag23900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 22 open reading frame 33
Synonyms Testis-expressed protein 33, Protein EAN57, EAN57, CIMIP4, Ciliary microtubule inner protein 4
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC042635
Gene Symbol C22orf33
Gene ID (NCBI) 339669
RRIDAB_2880558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43247
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C22orf33 antibody 26577-1-APDownload protocol
IF protocol for C22orf33 antibody 26577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols