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ERLEC1 Polyclonal antibody

ERLEC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 29773-1-AP

Print datasheet

Synonyms

C2orf30, CL24936, CL25084, Endoplasmic reticulum lectin 1, ER lectin, ERLEC1, Erlectin, XTP3 B, XTP3TPB

验证数据展示

  • WB analysis using 29773-1-AP

    WB analysis using 29773-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29773-1-AP (ERLEC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29773-1-AP

    IHC staining of mouse brain using 29773-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29773-1-AP

    IHC staining of mouse brain using 29773-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, mouse colon tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29773-1-AP targets ERLEC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ERLEC1 fusion protein Ag31135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 2 open reading frame 30
Synonyms C2orf30, CL24936, CL25084, Endoplasmic reticulum lectin 1, ER lectin, ERLEC1, Erlectin, XTP3 B, XTP3TPB
Calculated Molecular Weight 55 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberNM_015701
Gene Symbol ERLEC1
Gene ID (NCBI) 27248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DZ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERLEC1 antibody 29773-1-APDownload protocol
IHC protocol for ERLEC1 antibody 29773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols