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C2orf49 Polyclonal antibody

C2orf49 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18272-1-AP

Print datasheet

Synonyms

ASW, Ashwin

验证数据展示

  • WB analysis using 18272-1-AP

    WB analysis using 18272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18272-1-AP (C2orf49 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 18272-1-AP

    IF Staining of A431 using 18272-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 18272-1-AP (C2orf49 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, Jurkat cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18272-1-AP targets C2orf49 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C2orf49 fusion protein Ag12948 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 2 open reading frame 49
Synonyms ASW, Ashwin
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC001310
Gene Symbol C2orf49
Gene ID (NCBI) 79074
RRIDAB_2066525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C2orf49 antibody 18272-1-APDownload protocol
IF protocol for C2orf49 antibody 18272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols