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C2orf79/PTRHD1 Polyclonal antibody

C2orf79/PTRHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25576-1-AP

Print datasheet

Synonyms

PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29

验证数据展示

  • WB analysis of HepG2 using 25576-1-AP

    WB analysis of HepG2 using 25576-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25576-1-AP (C2orf79/PTRHD1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 25576-1-AP

    IP experiment of COLO 320 using 25576-1-AP

    IP result of anti-C2orf79/PTRHD1 (IP:25576-1-AP, 4ug; Detection:25576-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • IF Staining of HepG2 using 25576-1-AP

    IF Staining of HepG2 using 25576-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 25576-1-AP

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 25576-1-AP

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25576-1-AP targets C2orf79/PTRHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen C2orf79/PTRHD1 fusion protein Ag22255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 2 open reading frame 79
Synonyms PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29
Calculated Molecular Weight 140 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC073803
Gene Symbol PTRHD1
Gene ID (NCBI) 391356
RRIDAB_2880136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6GMV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IHC protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IF protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IP protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols