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验证数据展示

  • WB analysis of HepG2 using 25576-1-AP

    WB analysis of HepG2 using 25576-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25576-1-AP (C2orf79/PTRHD1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 25576-1-AP

    IP experiment of COLO 320 using 25576-1-AP

    IP result of anti-C2orf79/PTRHD1 (IP:25576-1-AP, 4ug; Detection:25576-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • IF Staining of HepG2 using 25576-1-AP

    IF Staining of HepG2 using 25576-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 25576-1-AP

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 25576-1-AP

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C2orf79/PTRHD1 Polyclonal antibody

C2orf79/PTRHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25576-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25576-1-AP targets C2orf79/PTRHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 C2orf79/PTRHD1 fusion protein Ag22255 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 2 open reading frame 79
别名 PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29
计算分子量 140 aa, 16 kDa
观测分子量 13-16 kDa
GenBank蛋白编号BC073803
基因名称 PTRHD1
Gene ID (NCBI) 391356
RRIDAB_2880136
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6GMV3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IHC protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IF protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IP protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols