- Featured Product
- KD/KO Validated
C3orf64 Polyclonal antibody
C3orf64 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (2)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27595-1-AP targets C3orf64 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, rat |
| Immunogen | C3orf64 fusion protein Ag25932 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromosome 3 open reading frame 64 |
| Synonyms | AER61, C3orf64, EGF domain-specific O-linked GlcNAc transferase, EOGT, EOGT1 |
| Observed Molecular Weight | 60-62 kDa |
| GenBank Accession Number | BC060887 |
| Gene Symbol | C3orf64 |
| Gene ID (NCBI) | 285203 |
| RRID | AB_2880919 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5NDL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C3orf64 antibody 27595-1-AP | Download protocol |
| IHC protocol for C3orf64 antibody 27595-1-AP | Download protocol |
| IP protocol for C3orf64 antibody 27595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Extracell Vesicles Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Genomics SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.
| ||
Proteomics Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs | ||
Cartilage HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes
|
