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C4-B Alpha Chain/C4b-B/C4d-B Polyclonal antibody

C4-B Alpha Chain/C4b-B/C4d-B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55228-1-AP

Print datasheet

Synonyms

C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3

验证数据展示

  • WB analysis of human blood using 55228-1-AP

    WB analysis of human blood using 55228-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 55228-1-AP (C4-B alpha chain/C4b-B/C4d-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman blood

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55228-1-AP targets C4-B Alpha Chain/C4b-B/C4d-B in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 4B (Chido blood group)
Synonyms C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 95 kDa, 300 kDa
GenBank Accession NumberNM_000592
Gene Symbol C4B
Gene ID (NCBI) 721
RRIDAB_2881291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C0L5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4-B protein can recognize C4-B precursor, C4-B alpha chain, C4b-B, and C4d-B.

实验方案

Product Specific Protocols
WB protocol for C4-B Alpha Chain/C4b-B/C4d-B antibody 55228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols