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C4 Gamma Chain Polyclonal antibody

C4 Gamma Chain Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17160-1-AP

Print datasheet

Synonyms

Acidic complement C4, C4, C4 (gamma chain), C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

验证数据展示

  • WB analysis of human plasma using 17160-1-AP

    WB analysis of human plasma using 17160-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17160-1-AP

    IHC staining of human liver cancer using 17160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17160-1-AP

    IHC staining of human liver cancer using 17160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17160-1-AP is C4 Gamma Chain Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human
Immunogen C4 Gamma Chain fusion protein Ag10449 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 4A (Rodgers blood group)
Synonyms Acidic complement C4, C4, C4 (gamma chain), C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG
Calculated Molecular Weight 1744 aa, 193 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC012372
Gene Symbol C4A
Gene ID (NCBI) 720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C0L4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4A protein recognizes the C4 gamma chain.

实验方案

Product Specific Protocols
WB protocol for C4 Gamma Chain antibody 17160-1-APDownload protocol
IHC protocol for C4 Gamma Chain antibody 17160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols