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C4orf49 Polyclonal antibody

C4orf49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22569-1-AP

Print datasheet

Synonyms

C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP

验证数据展示

  • WB analysis of A2780 using 22569-1-AP

    WB analysis of A2780 using 22569-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22569-1-AP

    IHC staining of human testis using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

  • IHC staining of human ovary using 22569-1-AP

    IHC staining of human ovary using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inA2780 cells, A549 cells, SKOV-3 cells
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22569-1-AP targets C4orf49 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C4orf49 fusion protein Ag16552 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 4 open reading frame 49
Synonyms C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberBC104174
Gene Symbol C4orf49
Gene ID (NCBI) 84709
RRIDAB_2879124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).

实验方案

Product Specific Protocols
WB protocol for C4orf49 antibody 22569-1-APDownload protocol
IHC protocol for C4orf49 antibody 22569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols