验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, A549 cells, SKOV-3 cells |
Positive IHC detected in | human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22569-1-AP targets C4orf49 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | C4orf49 fusion protein Ag16552 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 4 open reading frame 49 |
别名 | C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP |
计算分子量 | 240 aa, 25 kDa |
观测分子量 | 33-36 kDa |
GenBank蛋白编号 | BC104174 |
基因名称 | C4orf49 |
Gene ID (NCBI) | 84709 |
RRID | AB_2879124 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TDB4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).
实验方案
Product Specific Protocols | |
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WB protocol for C4orf49 antibody 22569-1-AP | Download protocol |
IHC protocol for C4orf49 antibody 22569-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |