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C5 Polyclonal antibody

C5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22492-1-AP

Print datasheet

Synonyms

C5, C5b, C5, C5a, Complement C5 alpha chain, Complement C5, C5a anaphylatoxin

验证数据展示

  • WB analysis using 22492-1-AP

    WB analysis using 22492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma, rat plasma, mouse serum

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22492-1-AP targets C5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C5 fusion protein Ag17882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 5
Synonyms C5, C5b, C5, C5a, Complement C5 alpha chain, Complement C5, C5a anaphylatoxin
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight120 kDa, 70 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP01031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)

实验方案

Product Specific Protocols
WB protocol for C5 antibody 22492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols