验证数据展示
经过测试的应用
Positive WB detected in | U-937 cells, U-87 MG cells |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84336-6-RR targets C5AR1/CD88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | FusionProtein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | complement component 5a receptor 1 |
别名 | C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1 |
计算分子量 | 39kDa |
GenBank蛋白编号 | NM_001736.4 |
基因名称 | C5aR |
Gene ID (NCBI) | 728 |
RRID | AB_3671877 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P21730 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).
实验方案
Product Specific Protocols | |
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WB protocol for C5AR1/CD88 antibody 84336-6-RR | Download protocol |
IF protocol for C5AR1/CD88 antibody 84336-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |