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验证数据展示

  • WB analysis using 84336-6-RR

    WB analysis using 84336-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84336-6-RR (C5AR1/CD88 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 84336-6-RR

    IF Staining of THP-1 using 84336-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C5AR1/CD88 Recombinant antibody

C5AR1/CD88 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84336-6-RR

Print datasheet

CloneNo. 241712G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, U-87 MG cells
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy C5AR1 Ready-To-Use IHC Kit. C5AR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84336-6-RR targets C5AR1/CD88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement component 5a receptor 1
Synonyms C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1
Calculated Molecular Weight39kDa
GenBank Accession NumberNM_001736.4
Gene Symbol C5aR
Gene ID (NCBI) 728
RRIDAB_3671877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

实验方案

Product Specific Protocols
WB protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
IF protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols