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  • Featured Product
  • KD/KO Validated

C5orf33 Polyclonal antibody

C5orf33 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26352-1-AP

Print datasheet

Synonyms

NAD kinase domain-containing protein 1, mitochondrial, NAD kinase 2, mitochondrial, NAD kinase 2, MNADK, Mitochondrial NAD kinase

验证数据展示

  • WB analysis of mouse liver using 26352-1-AP

    WB analysis of mouse liver using 26352-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26352-1-AP

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26352-1-AP

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 26352-1-AP

    IF Staining of HeLa using 26352-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26352-1-AP (C5orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, HL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26352-1-AP targets C5orf33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen C5orf33 fusion protein Ag23706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 5 open reading frame 33
Synonyms NAD kinase domain-containing protein 1, mitochondrial, NAD kinase 2, mitochondrial, NAD kinase 2, MNADK, Mitochondrial NAD kinase
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062567
Gene Symbol C5orf33
Gene ID (NCBI) 133686
RRIDAB_2880487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4G0N4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C5orf33 antibody 26352-1-APDownload protocol
IHC protocol for C5orf33 antibody 26352-1-APDownload protocol
IF protocol for C5orf33 antibody 26352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

bioRxiv

Mitochondrial NADK2-dependent NADPH controls Tau oligomer uptake in human neurons

Authors - Evelyn Pardo
  • KD Validated
View Article
humanWB

FASEB J

G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation

Authors - Teng Wu
View Article