C6 Polyclonal antibody
C6 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human blood |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17239-1-AP targets C6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | C6 fusion protein Ag11083 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | complement component 6 |
| Synonyms | C6, complement component 6, Complement component C6 |
| Calculated Molecular Weight | 934 aa, 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC035723 |
| Gene Symbol | C6 |
| Gene ID (NCBI) | 729 |
| RRID | AB_2878366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C6 antibody 17239-1-AP | Download protocol |
| IHC protocol for C6 antibody 17239-1-AP | Download protocol |
| IP protocol for C6 antibody 17239-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury. | ||
Oral Dis Analysis of Salivary Exosomal Proteins in Young Adults with Severe Periodontitis. | ||
Ann Diagn Pathol The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder | ||
Ren Fail Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury. | ||
Ann Diagn Pathol The amplification of CNS damage in Alzheimer's disease due to SARS-CoV2 infection | ||
Nat Commun A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells |
