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C6orf141 Polyclonal antibody

C6orf141 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24355-1-AP

Print datasheet

Synonyms

C6orf141

验证数据展示

  • WB analysis of A431 using 24355-1-AP

    WB analysis of A431 using 24355-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24355-1-AP (C6orf141 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24355-1-AP

    IHC staining of human breast cancer using 24355-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24355-1-AP (C6orf141 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 24355-1-AP

    IHC staining of human breast cancer using 24355-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24355-1-AP (C6orf141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24355-1-AP targets C6orf141 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen C6orf141 fusion protein Ag19668 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 141
Synonyms C6orf141
Calculated Molecular Weight 244 aa, 27 kDa
Observed Molecular Weight 27-31 kDa
GenBank Accession NumberBC036917
Gene Symbol C6orf141
Gene ID (NCBI) 135398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SZD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C6orf141 antibody 24355-1-APDownload protocol
IHC protocol for C6orf141 antibody 24355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols