C6orf15 Polyclonal antibody

C6orf15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24953-1-AP

Print datasheet

Synonyms

UNQ1840/PRO3566, STG, Protein STG



经过测试的应用

Positive WB detected inA375 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24953-1-AP targets C6orf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen C6orf15 fusion protein Ag20276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 15
Synonyms UNQ1840/PRO3566, STG, Protein STG
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC153157
Gene Symbol C6orf15
Gene ID (NCBI) 29113
RRIDAB_2879818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.

实验方案

Product Specific Protocols
WB protocol for C6orf15 antibody 24953-1-APDownload protocol
IHC protocol for C6orf15 antibody 24953-1-APDownload protocol
IF protocol for C6orf15 antibody 24953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cancer Cell Int

C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer

Authors - Jiankang Yu
humanIHC

Pathol Res Pract

C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer

Authors - Xiaoyu Xiong
humanIHC

Ann Diagn Pathol

Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein

Authors - Leilei Liu