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C6orf182 Polyclonal antibody

C6orf182 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24957-1-AP

Print datasheet

Synonyms

Cep57R, CEP57L1, Cep57 related protein, Centrosomal protein of 57 kDa-related protein, Centrosomal protein CEP57L1

验证数据展示

  • WB analysis using 24957-1-AP

    WB analysis using 24957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24957-1-AP (C6orf182 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24957-1-AP

    IF Staining of HeLa using 24957-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C6orf182 antibody (24957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, mouse kidney tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

24957-1-AP targets C6orf182 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, monkey
Cited Reactivityhuman
Immunogen C6orf182 fusion protein Ag21054 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 182
Synonyms Cep57R, CEP57L1, Cep57 related protein, Centrosomal protein of 57 kDa-related protein, Centrosomal protein CEP57L1
Calculated Molecular Weight 460 aa, 54 kDa
Observed Molecular Weight 55-65 kda
GenBank Accession NumberBC064365
Gene Symbol C6orf182
Gene ID (NCBI) 285753
RRIDAB_2879821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C6orf182 also named as Centrosomal protein of 57 kDa related protein is a 460 amino-acid protein, which belongs to translokin family. C6orf182 as centrosomal protein may be required for microtubule attachment to centrosomes and localizes in the cytoplasm. The function of C6orf182 need to be further studied.

实验方案

Product Specific Protocols
WB protocol for C6orf182 antibody 24957-1-APDownload protocol
IF protocol for C6orf182 antibody 24957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Cell Biol

Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.

Authors - Kei K Ito
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article