验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IF | See 3 publications below |
产品信息
24957-1-AP targets C6orf182 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, monkey |
| 文献引用反应性 | human |
| 免疫原 | C6orf182 fusion protein Ag21054 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 182 |
| 别名 | Cep57R, CEP57L1, Cep57 related protein, Centrosomal protein of 57 kDa-related protein, Centrosomal protein CEP57L1 |
| 计算分子量 | 460 aa, 54 kDa |
| 观测分子量 | 55-65 kda |
| GenBank蛋白编号 | BC064365 |
| 基因名称 | C6orf182 |
| Gene ID (NCBI) | 285753 |
| RRID | AB_2879821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYX8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C6orf182 also named as Centrosomal protein of 57 kDa related protein is a 460 amino-acid protein, which belongs to translokin family. C6orf182 as centrosomal protein may be required for microtubule attachment to centrosomes and localizes in the cytoplasm. The function of C6orf182 need to be further studied.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C6orf182 antibody 24957-1-AP | Download protocol |
| IF protocol for C6orf182 antibody 24957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle. | ||