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验证数据展示

  • WB analysis of MCF-7 using 25124-1-AP

    WB analysis of MCF-7 using 25124-1-AP

    WB result of C6orf211/ARMT1 antibody (25124-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C6orf211/ARMT1 transfected MCF-7 cells.

  • WB analysis of MCF-7 using 25124-1-AP

    WB analysis of MCF-7 using 25124-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25124-1-AP

    IHC staining of human breast cancer using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 25124-1-AP

    IHC staining of human breast cancer using 25124-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of T-47D using 25124-1-AP

    IF Staining of T-47D using 25124-1-AP

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using C6orf211/ARMT1 antibody (25124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  • KD/KO验证

C6orf211/ARMT1 Polyclonal antibody

C6orf211/ARMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25124-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

C6orf211, ARMT1, Sugar phosphate phosphatase ARMT1, EC:3.1.3.-, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, A431 cells, T-47D cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25124-1-AP targets C6orf211/ARMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C6orf211/ARMT1 fusion protein Ag19090 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 211
别名 C6orf211, ARMT1, Sugar phosphate phosphatase ARMT1, EC:3.1.3.-, EC:2.1.1.-
计算分子量 441 aa, 51 kDa
观测分子量 50-51 kDa
GenBank蛋白编号BC011348
基因名称 C6orf211/ARMT1
Gene ID (NCBI) 79624
RRIDAB_2879911
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H993
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
IHC protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
IF protocol for C6orf211/ARMT1 antibody 25124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols