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C7orf43 Polyclonal antibody

C7orf43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27790-1-AP

Print datasheet

Synonyms

C7orf43

验证数据展示

  • WB analysis using 27790-1-AP

    WB analysis using 27790-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27790-1-AP (C7orf43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27790-1-AP targets C7orf43 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen C7orf43 fusion protein Ag27149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 7 open reading frame 43
Synonyms C7orf43
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015722
Gene Symbol C7orf43
Gene ID (NCBI) 55262
RRIDAB_2880972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The predicted MW of C7orf43 is 62 kDa. Catalog#27790-1-AP recognises 65-70 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for C7orf43 antibody 27790-1-APDownload protocol
IHC protocol for C7orf43 antibody 27790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols