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C7orf58 Polyclonal antibody

C7orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20924-1-AP

Print datasheet

Synonyms

UNQ9432/PRO34713, CPED1, Cadherin-like and PC-esterase domain-containing protein 1

验证数据展示

  • WB analysis of A375 using 20924-1-AP

    WB analysis of A375 using 20924-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20924-1-AP

    IHC staining of human stomach using 20924-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human stomach using 20924-1-AP

    IHC staining of human stomach using 20924-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A375 using 20924-1-AP

    IF Staining of A375 using 20924-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using C7orf58 antibody (20924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA375 cells, A431 cells, HEK-293 cells, HeLa cells, L02 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20924-1-AP targets C7orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C7orf58 fusion protein Ag15193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 7 open reading frame 58
Synonyms UNQ9432/PRO34713, CPED1, Cadherin-like and PC-esterase domain-containing protein 1
Calculated Molecular Weight 1026 aa, 117 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC030538
Gene Symbol C7orf58
Gene ID (NCBI) 79974
RRIDAB_10697813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA4D0V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C7orf58 antibody 20924-1-APDownload protocol
IHC protocol for C7orf58 antibody 20924-1-APDownload protocol
IF protocol for C7orf58 antibody 20924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis

Authors - Siwei Pan
View Article