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C8orf4 Polyclonal antibody

C8orf4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26279-1-AP

Print datasheet

Synonyms

C8orf4, hTC 1, TC 1, TC1, Thyroid cancer protein 1, Uncharacterized protein C8orf4

验证数据展示

  • WB analysis of mouse kidney using 26279-1-AP

    WB analysis of mouse kidney using 26279-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26279-1-AP (C8orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26279-1-AP

    IHC staining of human thyroid cancer using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 26279-1-AP

    IHC staining of human thyroid cancer using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman thyroid cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

26279-1-AP targets C8orf4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C8orf4 fusion protein Ag23930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 8 open reading frame 4
Synonyms C8orf4, hTC 1, TC 1, TC1, Thyroid cancer protein 1, Uncharacterized protein C8orf4
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC021672
Gene Symbol C8orf4
Gene ID (NCBI) 56892
RRIDAB_2880460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR00
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C8orf4 antibody 26279-1-APDownload protocol
IHC protocol for C8orf4 antibody 26279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Medicine (Baltimore)

Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma

Authors - Gang Ren
View Article