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验证数据展示

  • WB analysis of human plasma using 25891-1-AP

    WB analysis of human plasma using 25891-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 25891-1-AP (C9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

C9 Polyclonal antibody

C9 Polyclonal Antibody for WB, ELISA
Cat No. 25891-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C9, complement component 9, Complement component C9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25891-1-AP targets C9 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen C9 fusion protein Ag23137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 9
Synonyms C9, complement component 9, Complement component C9
Calculated Molecular Weight 559 aa, 63 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC020721
Gene Symbol C9
Gene ID (NCBI) 735
RRIDAB_3085827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406).

实验方案

Product Specific Protocols
WB protocol for C9 antibody 25891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols