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验证数据展示

  • WB analysis of HepG2 using 23168-1-AP

    WB analysis of HepG2 using 23168-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 23168-1-AP

    IF Staining of HepG2 using 23168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFAF/EQTN antibody (23168-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

AFAF/EQTN Polyclonal antibody

AFAF/EQTN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23168-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

AFAF, C9orf11, EQTN, Equatorin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23168-1-AP targets AFAF/EQTN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 AFAF/EQTN fusion protein Ag19656 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 11
别名 AFAF, C9orf11, EQTN, Equatorin
计算分子量 294 aa, 33 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC137351
基因名称 AFAF
Gene ID (NCBI) 54586
RRIDAB_2879222
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ60
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).

实验方案

Product Specific Protocols
WB protocol for AFAF/EQTN antibody 23168-1-APDownload protocol
IF protocol for AFAF/EQTN antibody 23168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols