验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
24068-1-AP targets C9orf41/CARNMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | C9orf41/CARNMT1 fusion protein Ag21268 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 9 open reading frame 41 |
| 别名 | C9orf41, EC:2.1.1.22, Carnosine N-methyltransferase, CARNMT1 |
| 计算分子量 | 409 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC034033 |
| 基因名称 | C9orf41 |
| Gene ID (NCBI) | 138199 |
| RRID | AB_2879425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q8N4J0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf41/CARNMT1 antibody 24068-1-AP | Download protocol |
| IF protocol for C9orf41/CARNMT1 antibody 24068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
