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C9orf61 Polyclonal antibody

C9orf61 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25630-1-AP

Print datasheet

Synonyms

C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123

验证数据展示

  • WB analysis of mouse skeletal muscle using 25630-1-AP

    WB analysis of mouse skeletal muscle using 25630-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25630-1-AP is C9orf61 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C9orf61 fusion protein Ag22242 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 9 open reading frame 61
Synonyms C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC113685
Gene Symbol C9orf61
Gene ID (NCBI) 9413
RRIDAB_2880170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for C9orf61 antibody 25630-1-APDownload protocol
IHC protocol for C9orf61 antibody 25630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP

Tissue Cell

FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins

Authors - Jiakun Liu
View Article