验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, SH-SY5Y cells |
| Positive IHC detected in | human appendicitis tissue, human ovary tumor tissue, human testis tissue, mouse kidney tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1500 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25757-1-AP targets C9orf72 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | C9orf72 fusion protein Ag22723 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 9 open reading frame 72 |
| 别名 | Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT |
| 计算分子量 | 481 aa, 54 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC068445 |
| 基因名称 | C9orf72 |
| Gene ID (NCBI) | 203228 |
| RRID | AB_2784548 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LT7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. This antibody detects the N-terminal of C9orf72.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf72 antibody 25757-1-AP | Download protocol |
| IHC protocol for C9orf72 antibody 25757-1-AP | Download protocol |
| IF protocol for C9orf72 antibody 25757-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. | ||
Acta Neuropathol An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD. | ||
Elife Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. | ||
Theranostics C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α | ||
Front Cell Neurosci Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation | ||
EMBO Rep C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains
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