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C9orf80 Polyclonal antibody

C9orf80 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26127-1-AP

Print datasheet

Synonyms

SOSS C, Sensor of ssDNA subunit C, INIP, hSSBIP1, HSPC291

验证数据展示

  • IHC staining of mouse brain using 26127-1-AP

    IHC staining of mouse brain using 26127-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26127-1-AP (C9orf80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26127-1-AP

    IHC staining of mouse brain using 26127-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26127-1-AP (C9orf80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 26127-1-AP

    IF Staining of U2OS using 26127-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C9orf80 antibody (26127-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26127-1-AP targets C9orf80 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C9orf80 fusion protein Ag23582 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 9 open reading frame 80
Synonyms SOSS C, Sensor of ssDNA subunit C, INIP, hSSBIP1, HSPC291
GenBank Accession NumberBC014881
Gene Symbol C9orf80
Gene ID (NCBI) 58493
RRIDAB_3669523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRY2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf80 (INIP) is also a nuclear protein related to protein synthesis and transportation. C9orf80 is a member of the core hSSB1 complex, which plays an important role in DNA damage response and genome stability maintenance(PMID: 23986477).

实验方案

Product Specific Protocols
IHC protocol for C9orf80 antibody 26127-1-APDownload protocol
IF protocol for C9orf80 antibody 26127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols