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C9orf86 Polyclonal antibody

C9orf86 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20848-1-AP

Print datasheet

Synonyms

RBEL1, Rab-like protein 6, Rab-like protein 1, pp8875, Partner of ARF

验证数据展示

  • WB analysis of MDA-MB-453s using 20848-1-AP

    WB analysis of MDA-MB-453s using 20848-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP (C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20848-1-AP

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20848-1-AP targets C9orf86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C9orf86 fusion protein Ag14350 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 9 open reading frame 86
Synonyms RBEL1, Rab-like protein 6, Rab-like protein 1, pp8875, Partner of ARF
Calculated Molecular Weight 729 aa, 80 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC002945
Gene Symbol C9orf86
Gene ID (NCBI) 55684
RRIDAB_2878750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3YEC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]

实验方案

Product Specific Protocols
WB protocol for C9orf86 antibody 20848-1-APDownload protocol
IHC protocol for C9orf86 antibody 20848-1-APDownload protocol
IF protocol for C9orf86 antibody 20848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Authors - Ning Wang
View Article