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Carbonic anhydrase 1/CA1 Polyclonal antibody

Carbonic anhydrase 1/CA1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13198-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

CA1, CA I, Carbonate dehydratase I, Car1, CA-I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse spleen tissue, Jurkat cells, rat spleen tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CA1 Ready-To-Use IHC Kit. CA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13198-2-AP targets Carbonic anhydrase 1/CA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Carbonic anhydrase 1/CA1 fusion protein Ag3932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonic anhydrase I
别名 CA1, CA I, Carbonate dehydratase I, Car1, CA-I
计算分子量 261 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC027890
基因名称 CA1
Gene ID (NCBI) 759
RRIDAB_2275041
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00915
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

实验方案

Product Specific Protocols
WB protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-APDownload protocol
IHC protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-APDownload protocol
IP protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB,IF

Acta Neuropathol Commun

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder

Authors - Ke Cheng
  • KO Validated
ratWB

J Ginseng Res

Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway

Authors - Mei-Li Lu
mouseIF

FASEB J

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins
humanWB

J Inflamm Res

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

Authors - Xianqin Song
mouseWB

Am J Physiol Lung Cell Mol Physiol

Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.

Authors - Kathleen Boyne
human,ratWB

Front Pharmacol

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Authors - Meili Lu
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