验证数据展示
经过测试的应用
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
12953-1-AP targets CA10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CA10 fusion protein Ag3652 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | carbonic anhydrase X |
| Synonyms | CA RP X, CA RPX, CA10, carbonic anhydrase X, CARP X, CARPX, Cerebral protein 15, HUCEP 15, UNQ533/PRO1076 |
| Calculated Molecular Weight | 328 aa, 38 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC020577 |
| Gene Symbol | CA10 |
| Gene ID (NCBI) | 56934 |
| RRID | AB_2877897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NS85 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CA10 antibody 12953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |