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验证数据展示

  • WB analysis of human kidney using 15435-1-AP

    WB analysis of human kidney using 15435-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 15435-1-AP

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 15435-1-AP

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

CA11 Polyclonal antibody

CA11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15435-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15435-1-AP targets CA11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CA11 fusion protein Ag7712 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase XI
Synonyms CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kda, 45 kDa
GenBank Accession NumberBC002662
Gene Symbol CA11
Gene ID (NCBI) 770
RRIDAB_2065700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75493
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

实验方案

Product Specific Protocols
WB protocol for CA11 antibody 15435-1-APDownload protocol
IHC protocol for CA11 antibody 15435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols