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验证数据展示

  • WB analysis of human kidney using 15435-1-AP

    WB analysis of human kidney using 15435-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 15435-1-AP

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 15435-1-AP

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

CA11 Polyclonal antibody

CA11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15435-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15435-1-AP targets CA11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CA11 fusion protein Ag7712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonic anhydrase XI
别名 CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1
计算分子量 36 kDa
观测分子量 36 kda, 45 kDa
GenBank蛋白编号BC002662
基因名称 CA11
Gene ID (NCBI) 770
RRIDAB_2065700
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75493
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

实验方案

Product Specific Protocols
WB protocol for CA11 antibody 15435-1-APDownload protocol
IHC protocol for CA11 antibody 15435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols