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CA11 Recombinant antibody, PBS Only

CA11 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230390F7

Cat no : 83082-4-PBS

Print datasheet

Synonyms

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

验证数据展示

  • IF Staining of U2OS using 83082-4-RR (same clone as 83082-4-PBS)

    IF Staining of U2OS using 83082-4-RR (same clone as 83082-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CA11 antibody (83082-4-RR, Clone: 230390F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83082-4-RR (same clone as 83082-4-PBS)

    FC experiment of A431 using 83082-4-RR (same clone as 83082-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CA11 Recombinant antibody (83082-4-RR, Clone:230390F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83082-4-RR

    Affinity and Kinetic Characterization of 83082-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-4-RR against Human CA11 were performed. The affinity constant is 0.78 nM.

产品信息

83082-4-PBS targets CA11 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen CA11 fusion protein Ag33373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carbonic anhydrase XI
Synonyms Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC002662
Gene Symbol CA11
Gene ID (NCBI) 770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75493
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).