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CA14 Polyclonal antibody

CA14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13736-1-AP

Print datasheet

Synonyms

CA XIV, CA14, Carbonate dehydratase XIV, Carbonic anhydrase 14, carbonic anhydrase XIV

验证数据展示

  • WB analysis using 13736-1-AP

    WB analysis using 13736-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13736-1-AP

    WB analysis using 13736-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse eye using 13736-1-AP

    WB analysis of mouse eye using 13736-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse eye tissue, mouse retina tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13736-1-AP targets CA14 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen CA14 fusion protein Ag4673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase XIV
Synonyms CA XIV, CA14, Carbonate dehydratase XIV, Carbonic anhydrase 14, carbonic anhydrase XIV
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC034412
Gene Symbol CA14
Gene ID (NCBI) 23632
RRIDAB_2065836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbonic anhydrase XIV (CAXIV, Car14) is highly expressed in the hepatocyte with predominance in the canalicular membrane and its active site in the extracellular milieu. The CA XIV isoform may also be of importance for regulation of neuronal excitability and transport processes in brain.

实验方案

Product Specific Protocols
WB protocol for CA14 antibody 13736-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Glia

Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome

Authors - Sophie B Siems
View Article