CA4 Polyclonal antibody

CA4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

Carbonic anhydrase 4, Carbonate dehydratase IV, Car4, CA-IV, CAIV

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13931-1-AP targets CA4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CA4 fusion protein Ag4960 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonic anhydrase IV
别名 Carbonic anhydrase 4, Carbonate dehydratase IV, Car4, CA-IV, CAIV
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC057792
基因名称 CA4
Gene ID (NCBI) 762
RRIDAB_2877990
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

实验方案

Product Specific Protocols
WB protocol for CA4 antibody 13931-1-APDownload protocol
IHC protocol for CA4 antibody 13931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Theranostics

Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype

Authors - Zhi-Jie Gao
WB

Sci Rep

Carbonic anhydrase IV in lizard chemical signals

Authors - Marco Mangiacotti
humanWB

J Cancer

Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.

Authors - Wanpeng Wang
humanIHC

Nat Commun

A CCL2+DPP4+ subset of mesenchymal stem cells expedites aberrant formation of creeping fat in humans

Authors - Fengfei Wu
humanIF

Am J Respir Cell Mol Biol

Activated Clec4nhi Neutrophils Aggravate Lung Injury in an Endothelial IGFBP7 Dependent Manner

Authors - Linchao Yu
Loading...