验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
13342-1-AP targets CA5B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | CA5B fusion protein Ag4166 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | carbonic anhydrase VB, mitochondrial |
别名 | CA VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB |
计算分子量 | 317 aa, 36 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC028142 |
基因名称 | CA5B |
Gene ID (NCBI) | 11238 |
RRID | AB_2243612 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y2D0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CA5B(Carbonic anhydrase 5B, mitochondrial) is also named as CA-VB and belongs to the alpha-carbonic anhydrase family.CA5B encodes a 317-amino acid protein that has 64% sequence similarity with CA5 (114761), a mitochondrial CA, and contains a 33-amino-acid residue signal sequence. Western blot analysis revealed a 36-kD protein precursor and a 32-kD mature protein(PMID:10409679).
实验方案
Product Specific Protocols | |
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WB protocol for CA5B antibody 13342-1-AP | Download protocol |
IHC protocol for CA5B antibody 13342-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |