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验证数据展示

  • IHC staining of human kidney using 13342-1-AP

    IHC staining of human kidney using 13342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13342-1-AP (CA5B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 13342-1-AP

    IHC staining of human kidney using 13342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13342-1-AP (CA5B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 13342-1-AP

    WB analysis of mouse testis using 13342-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13342-1-AP (CA5B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CA5B Polyclonal antibody

CA5B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13342-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CA VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13342-1-AP targets CA5B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CA5B fusion protein Ag4166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase VB, mitochondrial
Synonyms CA VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB
Calculated Molecular Weight 317 aa, 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC028142
Gene Symbol CA5B
Gene ID (NCBI) 11238
RRIDAB_2243612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2D0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA5B(Carbonic anhydrase 5B, mitochondrial) is also named as CA-VB and belongs to the alpha-carbonic anhydrase family.CA5B encodes a 317-amino acid protein that has 64% sequence similarity with CA5 (114761), a mitochondrial CA, and contains a 33-amino-acid residue signal sequence. Western blot analysis revealed a 36-kD protein precursor and a 32-kD mature protein(PMID:10409679).

实验方案

Product Specific Protocols
WB protocol for CA5B antibody 13342-1-APDownload protocol
IHC protocol for CA5B antibody 13342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Non-redundant roles of DIAPHs in primary ciliogenesis.

Authors - Oliva Palander
View Article