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CA7 Polyclonal antibody

CA7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13670-1-AP

Print datasheet

Synonyms

CA-VII, CAVII, carbonic anhydrase VII, Carbonic anhydrase 7, Carbonate dehydratase VII

验证数据展示

  • WB analysis using 13670-1-AP

    WB analysis using 13670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13670-1-AP

    IF Staining of HeLa using 13670-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 13670-1-AP

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13670-1-AP

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13670-1-AP targets CA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen CA7 fusion protein Ag4649 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase VII
Synonyms CA-VII, CAVII, carbonic anhydrase VII, Carbonic anhydrase 7, Carbonate dehydratase VII
Calculated Molecular Weight 264 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC033865
Gene Symbol CA7
Gene ID (NCBI) 766
RRIDAB_2243629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CA7 antibody 13670-1-APDownload protocol
IHC protocol for CA7 antibody 13670-1-APDownload protocol
IF protocol for CA7 antibody 13670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouse,rat,pigIF

Cell Regen

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium.

Authors - Haonan Li
View Article