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验证数据展示

  • IHC staining of mouse stomach using 84233-1-RR

    IHC staining of mouse stomach using 84233-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CA9 antibody included
  • Affinity and Kinetic Characterization of 84233-1-RR

    Affinity and Kinetic Characterization of 84233-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84233-1-RR against Human CA9 were performed. The affinity constant is below 1 pM.

Carbonic Anhydrase IX/CA9 Recombinant antibody

Carbonic Anhydrase IX/CA9 Uni-rAbTM Recombinant Antibody for IHC, ELISA
Cat No. 84233-1-RR

Print datasheet

CloneNo. 241573A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

CAIX, CA9, carbonic anhydrase IX, CA-IX, CA IX

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CA9 Ready-To-Use IHC Kit. CA9 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84233-1-RR targets Carbonic Anhydrase IX/CA9 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Carbonic Anhydrase IX/CA9 fusion protein Ag36941 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carbonic anhydrase IX
Synonyms CAIX, CA9, carbonic anhydrase IX, CA-IX, CA IX
Calculated Molecular Weight 459 aa, 50 kDa
GenBank Accession NumberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
RRIDAB_3671786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. (PMID: 31819036).

实验方案

Product Specific Protocols
IHC protocol for Carbonic Anhydrase IX/CA9 antibody 84233-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols