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CA9 Recombinant antibody, PBS Only (Capture)

CA9 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241573G5

Cat no : 84233-3-PBS

Print datasheet

Synonyms

CAIX, carbonic anhydrase IX, Carbonate dehydratase IX, CA-IX, CA IX

验证数据展示

  • Sandwich ELISA standard curve of MP01136-1

    Sandwich ELISA standard curve of MP01136-1

    Sandwich ELISA standard curve of MP01136-1, Human CA9 Recombinant Matched Antibody Pair - PBS only. 84233-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36941. 84233-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP01136-1

    Sample test of MP01136-1

    HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CA9, and measured 2,951.1 pg/mL.

  • Sample test of MP01136-1

    Sample test of MP01136-1

    Urine of six individual healthy human donors was measured. Five measured less than the lowest human CA9 standard, 15.6 pg/mL. One sample read 47.2 pg/mL.

  • Sample test of MP01136-1

    Sample test of MP01136-1

    Serum of six individual healthy human donors was measured. The CA9 concentration of detected samples was determined to be 246.1 pg/mL with a range of 71.5-420.1 pg/mL.

  • Cytometric bead array standard curve of MP01136-1

    Cytometric bead array standard curve of MP01136-1

    Cytometric bead array standard curve of MP01136-1, CA9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84233-3-PBS. Detection antibody: 84233-5-PBS. Standard: Ag36941. Range: 0.313-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CA9 Ready-To-Use IHC Kit. CA9 primary antibody included.

产品信息

84233-3-PBS targets CA9 as part of a matched antibody pair:

MP01136-1: 84233-3-PBS capture and 84233-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen CA9 fusion protein Ag36941 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carbonic anhydrase IX
Synonyms CAIX, carbonic anhydrase IX, Carbonate dehydratase IX, CA-IX, CA IX
Calculated Molecular Weight 459 aa, 50 kDa
GenBank Accession NumberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16790
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C