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验证数据展示

  • WB analysis using 83350-4-RR

    WB analysis using 83350-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83350-4-RR (CACHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83350-4-RR

    Affinity and Kinetic Characterization of 83350-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83350-4-RR against Human CACHD1 were performed. The affinity constant is 6.83 nM.

CACHD1 Recombinant antibody

CACHD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83350-4-RR

产品说明书

CloneNo. 240197A2

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83350-4-RR targets CACHD1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 CACHD1 fusion protein Ag22138 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cache domain containing 1
别名 VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1
计算分子量 1274 aa, 142 kDa
观测分子量 140-170 kDa
GenBank蛋白编号BC039301
基因名称 CACHD1
Gene ID (NCBI) 57685
RRIDAB_3671008
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5VU97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACHD1 (Cache domain-containing protein 1), also called VWCD1 (VWFA and cache domain-containing protein 1), is a type I membrane protein involved that modulates the activities of voltage-gated Ca(2+) channels (VGCCs) (PMID: 30181139; 30404013). CACHD1 has two isoforms, with molecular weights of 142 kDa and 109 kDa respectively. It has certain glycosylation modifications, and the molecular weight of isoform1 after glycosylation modification is 170 kDa (PMID: 30983497; 30404013). This antibody recognizes 142-170 kDa.

实验方案

Product Specific Protocols
WB protocol for CACHD1 antibody 83350-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols