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验证数据展示

  • WB analysis of HEK-293 using 22276-1-AP

    WB analysis of HEK-293 using 22276-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22276-1-AP (CACNA1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 22276-1-AP

    IHC staining of human brain using 22276-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 22276-1-AP

    IHC staining of human brain using 22276-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 22276-1-AP

    IF Staining of SH-SY5Y using 22276-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 22276-1-AP (CACNA1D antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CACNA1D Polyclonal antibody

CACNA1D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CCHL1A2, CACNL1A2, CACN4, CACH3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22276-1-AP targets CACNA1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, L type, alpha 1D subunit
别名 CCHL1A2, CACNL1A2, CACN4, CACH3
计算分子量 248 kDa
观测分子量 200-250 kDa
GenBank蛋白编号NM_000720
基因名称 CACNA1D
Gene ID (NCBI) 776
RRIDAB_2879057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01668
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CACNA1D antibody 22276-1-APDownload protocol
IHC protocol for CACNA1D antibody 22276-1-APDownload protocol
IF protocol for CACNA1D antibody 22276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols