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CACNA1E Polyclonal antibody

CACNA1E Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27225-1-AP

Print datasheet

Synonyms

BII, Brain calcium channel II, CACH6, CACNA1E, CACNL1A6, Cav2.3

验证数据展示

  • IHC staining of human brain using 27225-1-AP

    IHC staining of human brain using 27225-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27225-1-AP

    IHC staining of human brain using 27225-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 27225-1-AP is CACNA1E Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen CACNA1E fusion protein Ag25944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms BII, Brain calcium channel II, CACH6, CACNA1E, CACNL1A6, Cav2.3
Calculated Molecular Weight 262 kDa
GenBank Accession NumberNM_000721
Gene Symbol CACNA1E
Gene ID (NCBI) 777
RRIDAB_2880808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CACNA1E antibody 27225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity.

Authors - Anita Siller
View Article