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CACNA1S Polyclonal antibody

CACNA1S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22279-1-AP

Print datasheet

Synonyms

CACH1, CACN1, CACNA1S, CACNL1A3, Cav1.1, CCHL1A3, HOKPP, hypoPP, MHS5

验证数据展示

  • WB analysis of human brain using 22279-1-AP

    WB analysis of human brain using 22279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22279-1-AP

    IHC staining of human skeletal muscle using 22279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 22279-1-AP

    IHC staining of human skeletal muscle using 22279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

22279-1-AP targets CACNA1S in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms CACH1, CACN1, CACNA1S, CACNL1A3, Cav1.1, CCHL1A3, HOKPP, hypoPP, MHS5
Calculated Molecular Weight 212 kDa
Observed Molecular Weight 200-220 kDa
GenBank Accession NumberNM_000069
Gene Symbol CACNA1S
Gene ID (NCBI) 779
RRIDAB_2879058
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13698
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.

实验方案

Product Specific Protocols
WB protocol for CACNA1S antibody 22279-1-APDownload protocol
IHC protocol for CACNA1S antibody 22279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Reproduction

Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.

Authors - Ru Zheng
View Article