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验证数据展示

  • IHC staining of mouse skeletal muscle using 18389-1-AP

    IHC staining of mouse skeletal muscle using 18389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 18389-1-AP

    IHC staining of mouse skeletal muscle using 18389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18389-1-AP

    IF Staining of HepG2 using 18389-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CACNG1 antibody (18389-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CACNG1 Polyclonal antibody

CACNG1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18389-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle calcium channel subunit gamma, CACNLG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18389-1-AP targets CACNG1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CACNG1 fusion protein Ag12918 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, gamma subunit 1
别名 Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle calcium channel subunit gamma, CACNLG
计算分子量 222 aa, 25 kDa
GenBank蛋白编号BC069493
基因名称 CACNG1
Gene ID (NCBI) 786
RRIDAB_2878540
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06432
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CACNG1 antibody 18389-1-APDownload protocol
IF protocol for CACNG1 antibody 18389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols