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验证数据展示

  • WB analysis of HEK-293 using 13729-1-AP

    WB analysis of HEK-293 using 13729-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13729-1-AP

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13729-1-AP

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNG3 Polyclonal antibody

CACNG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13729-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Voltage-dependent calcium channel gamma-3 subunit, Transmembrane AMPAR regulatory protein gamma-3, TARP gamma-3, Neuronal voltage-gated calcium channel gamma-3 subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13729-1-AP targets CACNG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CACNG3 fusion protein Ag4730 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, gamma subunit 3
别名 Voltage-dependent calcium channel gamma-3 subunit, Transmembrane AMPAR regulatory protein gamma-3, TARP gamma-3, Neuronal voltage-gated calcium channel gamma-3 subunit
计算分子量 315 aa, 36 kDa
观测分子量 40 kDa
GenBank蛋白编号BC040005
基因名称 CACNG3
Gene ID (NCBI) 10368
RRIDAB_2070085
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60359
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of alpha-1, beta, alpha-2/delta, and gamma subunits. CACNG3, one of the eight gamma subunits in human, is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors.

实验方案

Product Specific Protocols
WB protocol for CACNG3 antibody 13729-1-APDownload protocol
IHC protocol for CACNG3 antibody 13729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

Integrated profiling identifies CACNG3 as a prognostic biomarker for patients with glioma

Authors - Enfang Shan
View Article