验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32066-1-AP targets CACTIN in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CACTIN fusion protein Ag35645 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 29 |
| 别名 | Splicing factor Cactin, Renal carcinoma antigen NY-REN-24 |
| 计算分子量 | 108 kDa, 89 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_021231 |
| 基因名称 | C19orf29 |
| Gene ID (NCBI) | 58509 |
| RRID | AB_3670183 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WUQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CACTIN plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (PubMed:28062851).CACTIN was reported to be involved in the regulation of innate immune response (PubMed:20829348). CACTIN acts as a negative regulator of Toll-like receptor, interferon-regulatory factor (IRF) and canonical NF-kappa-B signaling pathways (PubMed:20829348, PubMed:26363554). CACTIN contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous pro-inflammatory stimuli (PubMed:20829348, PubMed:26363554). CACTIN has two isforms (89kDa and 108 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CACTIN antibody 32066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |