• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CACYBP Polyclonal antibody, PBS Only

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11745-1-PBS

Print datasheet

Synonyms

Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5

验证数据展示

  • WB analysis of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    WB analysis of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    IF Staining of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 11745-1-AP (same clone as 11745-1-PBS)

    IP experiment of mouse brain using 11745-1-AP (same clone as 11745-1-PBS)

    IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

    IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CACYBP antibody included
  • FC experiment of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    FC experiment of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CACYBP Ready-To-Use IHC Kit. CACYBP primary antibody included.

产品信息

11745-1-PBS targets CACYBP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CACYBP fusion protein Ag2340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcyclin binding protein
Synonyms Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
RRIDAB_2070266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB71
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).