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验证数据展示

  • IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

    IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

    IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CAD Polyclonal antibody, PBS Only

CAD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16617-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16617-1-PBS targets CAD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 CAD fusion protein Ag9912 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
别名
计算分子量 2225 aa, 243 kDa
观测分子量 240 kDa
GenBank蛋白编号BC014178
基因名称 CAD
Gene ID (NCBI) 790
RRIDAB_2878289
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27708
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).