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CAPS1 Polyclonal antibody

CAPS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11490-2-AP

Print datasheet

Synonyms

CADPS, CAPS, Calcium-dependent secretion activator 1, Calcium-dependent activator protein for secretion 1, Calcium dependent secretion activator 1

验证数据展示

  • IHC staining of human pancreas using 11490-2-AP

    IHC staining of human pancreas using 11490-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11490-2-AP (CAPS1 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 11490-2-AP

    IHC staining of human pancreas using 11490-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11490-2-AP (CAPS1 antibody at dilution of 1:200 (under 40x lens).

  • WB analysis of rat brain using 11490-2-AP

    WB analysis of rat brain using 11490-2-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11490-2-AP (CAPS1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 11490-2-AP

    IF Staining of SH-SY5Y using 11490-2-AP

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 11490-2-AP (CAPS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11490-2-AP targets CAPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CAPS1 fusion protein Ag1975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ca++-dependent secretion activator
Synonyms CADPS, CAPS, Calcium-dependent secretion activator 1, Calcium-dependent activator protein for secretion 1, Calcium dependent secretion activator 1
Calculated Molecular Weight 1353 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC015754
Gene Symbol CAPS1
Gene ID (NCBI) 8618
RRIDAB_2068173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPS1 antibody 11490-2-APDownload protocol
IHC protocol for CAPS1 antibody 11490-2-APDownload protocol
IF protocol for CAPS1 antibody 11490-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols