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验证数据展示

  • WB analysis using 22244-1-AP

    WB analysis using 22244-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of TT using 22244-1-AP

    IP experiment of TT using 22244-1-AP

    IP result of anti-CALCA/CGRP (IP:22244-1-AP, 4ug; Detection:22244-1-AP 1:600) with TT cells lysate 945 ug.

CALCA/Calcitonin Polyclonal antibody

CALCA/Calcitonin Polyclonal Antibody for WB, IP, ELISA
Cat No. 22244-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CALCA/CGRP, CALCA, Calcitonin, CALC1, Calcitonin gene-related peptide 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTT cells, Transfected HEK-293 cells
Positive IP detected inTT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22244-1-AP targets CALCA/Calcitonin in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 CALCA/Calcitonin fusion protein Ag17737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Calcitonin
别名 CALCA/CGRP, CALCA, Calcitonin, CALC1, Calcitonin gene-related peptide 1
计算分子量 141 aa, 15 kDa
观测分子量14 kDa, 5 kDa
GenBank蛋白编号BC093753
基因名称 CALCA
Gene ID (NCBI) 796
RRIDAB_3669393
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01258
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

实验方案

Product Specific Protocols
WB protocol for CALCA/Calcitonin antibody 22244-1-APDownload protocol
IP protocol for CALCA/Calcitonin antibody 22244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols