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CALCA/CGRP Monoclonal antibody

CALCA/CGRP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A1

Cat No : 68774-1-Ig

Print datasheet

Synonyms

Alpha type CGRP, CALC1, CALCA, CGRP, CGRP I, CGRP1, CT, KC

验证数据展示

  • WB analysis of TT using 68774-1-Ig

    WB analysis of TT using 68774-1-Ig

    TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 68774-1-Ig

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 68774-1-Ig

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inTT cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68774-1-Ig targets CALCA/CGRP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen CALCA/CGRP fusion protein Ag26141 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcitonin-related polypeptide alpha
Synonyms Alpha type CGRP, CALC1, CALCA, CGRP, CGRP I, CGRP1, CT, KC
Calculated Molecular Weight 141 aa, 15 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC093753
Gene Symbol CALCA
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).

实验方案

Product Specific Protocols
WB protocol for CALCA/CGRP antibody 68774-1-IgDownload protocol
IHC protocol for CALCA/CGRP antibody 68774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols