验证数据展示
经过测试的应用
Positive IF/ICC detected in | TT cells |
Positive FC (Intra) detected in | TT cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83774-1-RR targets CALCA/Calcitonin in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | Calcitonin |
别名 | Procalcitonin/CALCA, CALCA, Calcitonin, 240753G2, CALC1 |
计算分子量 | 15kDa |
GenBank蛋白编号 | NM_001741.3 |
基因名称 | CALCA |
Gene ID (NCBI) | 796 |
RRID | AB_3671370 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P01258 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.
实验方案
Product Specific Protocols | |
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IF protocol for CALCA/Calcitonin antibody 83774-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |