验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HT-1080 cells, HeLa cells, MCF-7 cells, Ramos cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, human kidney tissue, mouse brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
19843-1-AP targets CALCOCO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | CALCOCO1 fusion protein Ag13876 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium binding and coiled-coil domain 1 |
| 别名 | Sarcoma antigen NY-SAR-3, PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin |
| 计算分子量 | 691 aa, 77 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC003177 |
| 基因名称 | CALCOCO1 |
| Gene ID (NCBI) | 57658 |
| RRID | AB_10637265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P1Z2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| IHC protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| IF protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.
| ||
Cell Signal Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy. | ||
Nat Commun Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer |
